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In chemistry, the molar absorption coefficient or molar attenuation coefficient (ε) is a measurement of how strongly a chemical species absorbs, and thereby attenuates, light at a given wavelength. It is an intrinsic property of the species.
The spectrum of pure bilirubin is known, so the molar attenuation coefficient ε is known. Measurements of decadic attenuation coefficient μ 10 are made at one wavelength λ that is nearly unique for bilirubin and at a second wavelength in
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What is molar absorption coefficient?
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/ is the mass absorption coefficient is the molar mass in g/mol is Avogadro constant This is also commonly expressed as: = / where: is the absorption coefficient
The Molar attenuation coefficient (also called "molar absorptivity"), which is the absorption coefficient divided by molarity (see also Beer–Lambert law) The mass attenuation coefficient (also called "mass extinction coefficient"), which is the absorption coefficient divided by density
Absorption spectroscopy is spectroscopy that involves techniques that measure the absorption of electromagnetic radiation, as a function of frequency or wavelength, due to its interaction with a sample. The sample absorbs energy, i.e., photons, from the radiating field.
The mass attenuation coefficient can be thought of as a variant of absorption cross section where the effective area is defined per unit mass instead of per particle. Mathematical definitions. Mass attenuation coefficient is defined as. where. μ is the attenuation coefficient (linear attenuation coefficient); ρm is the mass density.
Thus, an Absorbance (A) of 1 corresponds to a concentration of 50 μg/mL for double-stranded DNA. This method of calculation is valid for up to an A of at least 2. A more accurate extinction coefficient may be needed for oligonucleotides; these can be predicted.
