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即時聚合酶連鎖反應 （英語： Real-time polymerase chain reaction ）是一種在 DNA 擴增反應中，以螢光染劑偵測每次 聚合酶鏈鎖反應 （PCR）循環後產物總量的方法 [1] 。 此實驗法已被眾多科學家採用，因為其偵測範圍廣、靈敏度高、準確、專一及快速。 PCR的發展因為偵測感染病患的病毒（不過RNA病毒須先反轉錄成DNA才進行PCR）或細菌量、癌症監測、診斷個別基因差異的用藥反應等應用而大幅提高。 PCR的方法是基於偵測目標物在反應前及PCR後產物的量化關係，越早探測到指定螢光值表示目標基因組越多。 相對於終端PCR方式（end-point PCR，傳統的PCR配合 凝膠電泳 ，在反應後偵測），qPCR（定量即時聚合酶連鎖反應）有許多優點。
A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR.
2022年12月16日 · Real-time polymerase chain reaction (real-time PCR), also known as quantitative PCR, is a modification of the PCR strategy which allows monitoring of the PCR progress in real-time PCR itself is an enzymatic process used in vitro for the amplification of a
RT-qPCR的簡介. 以RNA為起始材料. 當起始材料為RNA時，可使用定量反轉錄PCR (RT-qPCR)進行分析。 在這種方法中，RNA首先透過反轉錄酶（reverse transcriptase）從total RNA或messenger RNA（mRNA）轉錄成互補DNA（cDNA）。 之後cDNA將作為qPCR反應的模板。 RT-qPCR可用於各式應用，包括基因表現分析、RNAi驗證、微陣列驗證、病原體檢測、基因檢測和疾病研究。 一步法（One-step） vs. 兩步法（Two-step）RT-qPCR. RT-qPCR可以一步法（one-step）或者兩步法（two-step）檢測進行分析（圖1，表1）。
Real-time PCR/qPCR assays have become the tool of choice for the rapid and sensitive determination and quantitation of nucleic acid in various biological samples, with diverse applications such as gene expression analysis, the detection of genetically modified organisms in food, and cancer phenotyping.
2017年11月9日 · (Real-Time Quantitative Reverse Transcription PCR) is a major development of PCR technology that enables reliable detection and measurement of products generated during each cycle of PCR process. This technique became possible after introduction of an oligonucleotide probe which was designed to hybridize within the target sequence.
即時聚合酶鏈式反應 （英語： Real-time polymerase chain reaction ）是一種在 DNA 擴增反應中，以螢光染劑偵測每次 聚合酶鏈鎖反應 （PCR）循環後產物總量的方法 [1] 。 此實驗法已被眾多科學家採用，因為其偵測範圍廣、靈敏度高、準確、專一及快速。 PCR的發展因為偵測感染病患的病毒（不過RNA病毒須先反轉錄成DNA才進行PCR）或細菌量、癌症監測、診斷個別基因差異的用藥反應等應用而大幅提高。 PCR的方法是基於偵測目標物在反應前及PCR後產物的量化關係，越早探測到指定螢光值表示目標基因組越多。 相對於終端PCR方式（end-point PCR，傳統的PCR配合 凝膠電泳 ，在反應後偵測），qPCR（定量即時聚合酶鏈式反應）有許多優點。
Real-time PCR, also known as qPCR, is used for many qualitative and quantitative applications, including gene expression analysis, microRNA analysis for identification of cancer biomarkers, single nucleotide polymorphism (SNP) genotyping, copy number variation (CNV) analysis, and even protein analysis.
PCR Reagents and Enzymes›. 詳解 PCR 原理、步驟與循環參數—成功的六個關鍵考慮因素. PCR循環參數—成功的六個關鍵考量因素. ‹. 一旦 確定 DNA的適當用量和PCR (聚合酶連鎖反應)組成，即可以設定PCR循環和反應參數以進行有效擴增。. DNA聚合酶的特性 、PCR緩衝液類型 ...
Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected throughout the PCR process, rather than at the end of the PCR. This completely revolutionizes the way one approaches PCR-based quantitation of DNA and RNA.
